Parallel Artificial Membrane Permeability Assay (PAMPA) training video

Pion’s PAMPA technology uses a range of membranes to mimic gastrointestinal conditions or the blood-brain barrier or human skin. It allows you to evaluate the passive permeability of API's as well as the absorption potential of simple or complex formulations very precisely using robust models and protocols.

Parallel artificial membrane permeability assay (PAMPA) is a method which determines the permeability of substances from a donor compartment, through a lipid-infused artificial membrane into an acceptor compartment.  A multi-well plate is used for the donor and a membrane/acceptor compartment is placed on top; the whole assembly is commonly referred to as a “PAMPA sandwich.” At the beginning of the test, the drug is added to the donor compartment, and the acceptor compartment is drug-free. After an incubation period which may include stirring, the sandwich is separated and the amount of drug is measured in each compartment. Mass balance allows calculation of drug that remains in the membrane.

Bridging the gap between in vitro experiments and in vivo outcomes