Conserving biorelevant enzymatic activity during in vitro subcutaneous drug testing

We aim to advance preclinical research by improving in vitro-in vivo relationships(IVIVRs). Traditional in vitro methods often fall short of replicating the complexity of in vivo environments, especially for injectables, where the absence of key biomolecules at the injection site limits predictive accuracy. In this application note, we explain how we successfully integrated active enzymes into our in vitro analysis technique – the SCISSOR, enabling us to investigate pharmaceuticals that undergo enzyme-mediated transformations in vivo.

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