Real time monitoring the extent of plasmid-liposome complexation using 2nd derivative fiber-optic UV spectroscopy

In this application note we used Pion’s extensive experience in UV-Vis to revisit the use of the 2nd derivative spectroscopy for monitoring in real time structural changes and complexations of macromolecules (proteins and nucleic acids). This non-destructive method helps to overcome or reduce limitations of current measurement techniques such as a lag time between sample preparation and analysis as well as volume, mixing and concentration constraints.

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