A UV-Vis spectrophotometer is an essential tool in pharmaceutical development and quality control laboratories. It plays a critical role in identifying and quantifying chromophore-absorbing substances in solution, ensuring precision and accuracy in both research and manufacturing processes.
How Does a UV-Vis Spectrophotometer Work?
UV-Vis spectroscopy operates by passing electromagnetic energy through a sample. As this energy travels through the solution, the substance of interest absorbs a portion of the light within a specific wavelength range. The remaining light is then captured by a detector, which generates a unique absorbance spectrum for the sample.
The absorbance measurements obtained are compared to a calibration curve derived from known, traceable standards. This allows for accurate identification and quantification of the target substance within the sample.
It is important to note that for accurate results, the sample must be free of any undissolved particles, as the presence of such particles can cause light scattering, preventing accurate detection by the instrument.
Derivative Spectroscopy Analysis is a valid technique to solve the challenge of accurate measuring dynamic solubility samples with undissolved particles, in real-time as fast as every 5 seconds.
Our advanced Rainbow UV-Vis fiber optic probe dissolution monitoring system is widely trusted in pharmaceutical laboratories for its reliability and precision. Contact us today to learn more about how it can enhance your laboratory's performance and streamline your processes.
