Parallel Artificial Membrane Permeability Assay (PAMPA)
Parallel artificial membrane permeability assay
96 well plate-based permeability assay with membranes mimicking the gastrointestinal tract (GIT) or blood brain barrier (BBB) properties
Evaluate the passive permeability of API's as well as the absorption potential of simple or complex formulations.
Classification of API’s can be performed on BBB lipid for blood-brain barrier permeability in the CNS classification system
Permeability classification of API’s can be performed on GIT lipid to support the Biopharmaceutical Classification System (BCS) ranking
Configurations
PAMPA can be used for pre-formulation excipient screening:
To better mimic the GI absorption, PAMPA simulates the pH gradient between the gastrointestinal tract and blood plasma, which can affect the transport of ionizable molecules. In order to model transport conditions in the blood, the acceptor contains a scavenger binding molecule that helps to keep the free concentration of the API close to zero, leading to more accurate results.
Model Data
Bio-Mimetic Artificial Membrane Permeation Through:
Gastro Intestinal Tract (GIT)
GIT PAMPA vs. Human Jejunal Permeability
The GIT PAMPA (Double-Sink™) model data was initially developed against eight compounds evaluated at the University of Uppsala with human subjects. Since that time, additional data have been added, providing increased confidence in the model.
Blood Brain Barrier (BBB)
BBB PAMPA vs. Rodent Brain Perfusion
Nearly 40 CNS compounds were used for building the BBB PAMPA model.
The developed model can predict rodent brain perfusion uptake rate better than most more expensive cell-based assays in the market.
In the paper, Development and validation of PAMPA-BBB QSAR model to predict brain penetration potential of novel drug candidates, researchers tested ~2,000 small molecules to explore their permeability across the blood-brain barrier and correlated in vitro PAMPA-BBB data with in vivo brain permeation data in rodents to observe a categorical correlation of 77%, suggesting that models developed using data from PAMPA-BBB can forecast in vivo brain permeability.
Make the PAMPA System the Optimal Solution with Pion’s Gut-Box™
The Gut-Box™ was designed by Pion to complement our PAMPA Systems, resulting in the most comprehensive turn-key assays in the market for predicting in vivo permeability. Mount any 96 well plate (loaded with stirrers) on top of the Gut-Box™ and choose your stirring speed.
PAMPA Explorer Software
Our PAMPA Explorer Software takes off the burden of UV data analysis by using a proprietary UV spectrum shape analysis algorithm and also accurately calculates the API’s permeability on an easy-to-use interface.
Parallel Artificial Membrane Permeability Assay (PAMPA)
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