Pion’s PAMPA technology used a range of membranes to mimic gastro-intestinal conditions, or the blood-brain or human skin barrier. It allows you to evaluate the passive permeability of APIs as well as the absorption potential of simple or complex formulations very precisely using robust models and protocols.
Pion PAMPA has three key variants to support the permeability classification of APIs; GIT Lipid for gastrointestinal permeability of oral therapies using the Biopharmaceutical Classification System (BCS), BBB Lipid for blood-brain barrier permeability in the CNS classification system and SKIN PAMPA for dermal/transdermal penetration potential.
Pion PAMPA can be used for preformulation excipient screening;
Pion SKIN PAMPA offers a further ability for final formulation screening; comparing the performance of topical and transdermal formulations to rank order the permeability potential of those formulations.
PAMPA has a wide range of popular applications, each delivering robust and reliable results.
Our PAMPA Explorer Command Software is easy to interface, and is compatible out-of-the-box with the following commercially available UV readers and liquid handling robots:
Molecular Devices: SpectraMax® 190 • SpectraMax® Plus384 • SpectraMax® M5 • FlexStation® 3 • Tecan: Infinite® M200 • M1000 • BioTek: Epoch • Synergy 2/H4/HT/Mx/H1
PAMPA gives you the ability to screen the permeability of newly discovered APIs using a simple and robust model.
For example, PAMPA simulates the pH gradient between the gastrointestinal tract and blood plasma, which can affect the transport of ionizable molecules. In order to model transport conditions in the blood, the acceptor contains a scavenger binding molecule that helps to keep the free concentration of the API close to zero, leading to more accurate results.
While rodent in vivo and in situ studies of the kinetics of drug uptake across the gastro-intestinal in-terface, blood brain barrier, and via the skin are valuable assessment tools, they are relatively expen-sive and time consuming. PAMPA can reduce the number of animal studies using a system which mimics many of the properties of all these membranes.
Pion’s high throughput PAMPA method gives researchers a test system that is not only highly predic-tive of the human skin barrier, the blood-brain barrier, and the gastro-intestinal interface but also ex-peditious. Tests can be conducted manually or on robotic platforms for added flexibility.
We have developed a protocol on Double Sink PAMPA platform to study the effect of excipients on the solubility and permeability of APIs in a single assay. The gathered data could be used in early as-sessment of excipient influenced and pH-dependent GIT absorption. This could be beneficial in priori-tizing and perhaps minimizing the number of animal tests needed.
Drug ionization and physicochemical profiling
Investigation of the Efficacy of Transdermal Penetration Enhancers Through the Use of Human Skin and a Skin Mimic Artificial Membrane
P-Glycoprotein Deficient Mouse in situ Blood-Brain Barrier Permeability and its Prediction using an in combo PAMPA Model
The permeation of amphoteric drugs through artificial membranes - an in combo absorption model based on paracellular and transmembrane permeability
Coexistence of passive and active carrier-mediated uptake processes in drug transport: a more balanced view
Study on permeability enhancing effect of new ceramide-analogues by PAMPA method
Absorption-Excipient-pH Classification Gradient Maps: Sparingly-Soluble Drugs and the pH Partition Hypothesis
Membrane-water partitioning, membrane permeability, and baseline toxicity of the parasiticides ivermectin, albendazole, and morantel
Permeation of permanently charged molecules through artificial membranes - influence of physicochemical properties
PAMPA—critical factors for better predictions of absorption
PAMPA - a Drug Absorption in vitro Model. 13. Chemical Selectivity due to Membrane Hydrogen Bonding: in combo Comparisons of HDM-, DOPC-, and DS-PAMPA
HT solubility and permeability: MAD-PAMPA analysis. (in Pharmacokinetic Profiling in Drug Reasearch: Biological, Physicochemical, and Computational Strategies)
PAMPA-Excipient Classification Gradient Maps
Caco-2 Permeability of Weakly Basic Drugs Predicted with the Double-Sink PAMPA pKa flux Method
Advances in Screening for Membrane Permeability: High-Resolution PAMPA for Medicinal Chemists
The Rise of PAMPA
PAMPA - a drug absorption in vitro model. 7. Comparing rat in situ, Caco-2, and PAMPA permeability of fluoroquinolones
Absorption using the PAMPA Approach (in Optimization in Drug Discovery: In Vitro Methods)
PAMPA - a Drug Absorption in vitro Model. 8. Apparent Filter Porosity and the Unstirred Water Layer
PAMPA - a Drug Absorption in vitro Model. 11. Matching the in vivo Unstirred Water Layer Thickness by Individual-Well Stirring in Microtitre Plates
Acid-Base Cosolvent Method for Determining Aqueous Permeability of Amiodarone, Itraconazole, Tamoxifen, Terfenadine and Other Very Insoluble Molecules
In vitro permeability of poorly aqueous soluble compounds using different solubilizers in the PAMPA assay with liquid chromatography/mass spectrometry detection
PAMPA - a drug absorption in vitro model. 5. Unstirred water layer in iso-pH mapping assays and pKa flux - optimized design (pOD-PAMPA)
In vitro trans-monolayer permeability calculations: often forgotten assumption
High throughput physicochemical profiling for drug discovery
Permeability through DOPC/dodecane membranes: measurement and LFER modelling
A comparison of the in vitro permeation of niacinamide in mammalian skin and in the Parallel Artificial Membrane Permeation Assay (PAMPA) model
Studying transdermal patches using Skin PAMPA
Studying semi-solid formulations of diclofenac using Skin PAMPA
Application of Skin Pampa™ to Cosmetic Solvent Screening
Using Skin-PAMPA For Transdermal Patch Testing
Novel HT Method for Parallel Excipient/Vehicle Formulation Studies
Assessment of Transdermal Penetration Enhancement by Topical Pharmaceutical Excipients Using Skin PAMPA Method
Application of Skin PAMPA to Differentiate Between Topical Pharmaceutical Formulations of Ibuprofen
Skin-PAMPA: Fast Prediction of Skin Penetration
Skin-PAMPA: A New Method for Fast Prediction of Skin Penetration
Developing Skin PAMPA for Transdermal Patches
Evaluation of Optimized Caco-2 Permeability Measurements
Human Jejunal Permeability Predicted from Caco-2 Assay - A Biophysical Model Applied to Fluoroquinolone Antimicrobials
Very Thin PAMPA Membranes Indicate Higher Antipyrene Permeability but are Fragile and Contain Leaky Water Pores
Prediction of in situ Blood-Brain Barrier Permeability using BBB PAMPA Model
Drug Permeation in Skin PAMPA and Franz Cell Model
Penetration Enhancer Effect of Sucrose Esters
Novel in vitro PK (non-animal PK) Assay for Excipient/Vehicle Formulation Studies
Prediction of Caco-2 pH-Dependent Permeability based on High Quality in vitro Training Set
Pion's quick response time to our service needs keeps downtime to a minimum in our lab.