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PAMPA

Pion’s PAMPA technology used a range of membranes to mimic gastro-intestinal conditions, or the blood-brain or human skin barrier. It allows you to evaluate the passive permeability of APIs as well as the absorption potential of simple or complex formulations very precisely using robust models and protocols.

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Overview

Pion PAMPA has three key variants to support the permeability classification of APIs; GIT Lipid  for gastrointestinal permeability of oral therapies using the  Biopharmaceutical Classification System (BCS), BBB Lipid for blood-brain barrier permeability in the CNS classification system and SKIN PAMPA for dermal/transdermal penetration potential.

Pion  PAMPA can be used for preformulation excipient screening;

  • Comparative flux assay for smart excipient selection to enhance GI absorption
  • Comparative flux assay to select excipients for dermal/transdermal formulations.

Pion SKIN PAMPA offers a further ability for final formulation screening; comparing the performance of topical and transdermal formulations to rank order the permeability potential of those formulations.

Applications

PAMPA has a wide range of popular applications, each delivering robust and reliable results.

  • Study the simple solvent/excipient effect on flux
  • Measure passive permeability of compounds
  • Find the best excipients to help dermal absorption
  • Perform all evaluations using only modest sample quantities
  • Evaluate or compare complex formulations

Our PAMPA Explorer Command Software is easy to interface, and is compatible out-of-the-box with the following commercially available UV readers and liquid handling robots:

Molecular Devices: SpectraMax® 190 • SpectraMax® Plus384 • SpectraMax® M5 • FlexStation® 3 • Tecan: Infinite® M200 • M1000 • BioTek: Epoch • Synergy 2/H4/HT/Mx/H1

Features

Smart

PAMPA gives you the ability to screen the permeability of newly discovered APIs using a simple and robust model.

For example, PAMPA simulates the pH gradient between the gastrointestinal tract and blood plasma, which can affect the transport of ionizable molecules. In order to model transport conditions in the blood, the acceptor contains a scavenger binding molecule that helps to keep the free concentration of the API close to zero, leading to more accurate results.

Cost-effective

While rodent in vivo and in situ studies of the kinetics of drug uptake across the gastro-intestinal in-terface, blood brain barrier, and via the skin are valuable assessment tools, they are relatively expen-sive and time consuming. PAMPA can reduce the number of animal  studies  using a system which mimics many of the properties of all these membranes.

Time-saving

Pion’s high throughput PAMPA method gives researchers a test system that is not only highly predic-tive of the human skin barrier, the blood-brain barrier, and the gastro-intestinal interface but also ex-peditious. Tests can be conducted manually or on robotic platforms for added flexibility.

Pioneering

We have developed a protocol on Double Sink PAMPA platform to study the effect of excipients on the solubility and permeability of APIs in a single assay. The gathered data could be used in early as-sessment of excipient influenced and pH-dependent GIT absorption. This could be beneficial in priori-tizing and perhaps minimizing the number of animal tests needed.

Resources

Drug ionization and physicochemical profiling

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Investigation of the Efficacy of Transdermal Penetration Enhancers Through the Use of Human Skin and a Skin Mimic Artificial Membrane

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P-Glycoprotein Deficient Mouse in situ Blood-Brain Barrier Permeability and its Prediction using an in combo PAMPA Model

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The permeation of amphoteric drugs through artificial membranes - an in combo absorption model based on paracellular and transmembrane permeability

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Coexistence of passive and active carrier-mediated uptake processes in drug transport: a more balanced view

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Study on permeability enhancing effect of new ceramide-analogues by PAMPA method

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Absorption-Excipient-pH Classification Gradient Maps: Sparingly-Soluble Drugs and the pH Partition Hypothesis

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Membrane-water partitioning, membrane permeability, and baseline toxicity of the parasiticides ivermectin, albendazole, and morantel

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Permeation of permanently charged molecules through artificial membranes - influence of physicochemical properties

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PAMPA—critical factors for better predictions of absorption

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PAMPA - a Drug Absorption in vitro Model. 13. Chemical Selectivity due to Membrane Hydrogen Bonding: in combo Comparisons of HDM-, DOPC-, and DS-PAMPA

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HT solubility and permeability: MAD-PAMPA analysis. (in Pharmacokinetic Profiling in Drug Reasearch: Biological, Physicochemical, and Computational Strategies)

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PAMPA-Excipient Classification Gradient Maps

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Caco-2 Permeability of Weakly Basic Drugs Predicted with the Double-Sink PAMPA pKa flux Method

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Advances in Screening for Membrane Permeability: High-Resolution PAMPA for Medicinal Chemists

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The Rise of PAMPA

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PAMPA - a drug absorption in vitro model. 7. Comparing rat in situ, Caco-2, and PAMPA permeability of fluoroquinolones

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Absorption using the PAMPA Approach (in Optimization in Drug Discovery: In Vitro Methods)

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PAMPA - a Drug Absorption in vitro Model. 8. Apparent Filter Porosity and the Unstirred Water Layer

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PAMPA - a Drug Absorption in vitro Model. 11. Matching the in vivo Unstirred Water Layer Thickness by Individual-Well Stirring in Microtitre Plates

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Acid-Base Cosolvent Method for Determining Aqueous Permeability of Amiodarone, Itraconazole, Tamoxifen, Terfenadine and Other Very Insoluble Molecules

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In vitro permeability of poorly aqueous soluble compounds using different solubilizers in the PAMPA assay with liquid chromatography/mass spectrometry detection

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PAMPA - a drug absorption in vitro model. 5. Unstirred water layer in iso-pH mapping assays and pKa flux - optimized design (pOD-PAMPA)

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In vitro trans-monolayer permeability calculations: often forgotten assumption

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High throughput physicochemical profiling for drug discovery

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Permeability through DOPC/dodecane membranes: measurement and LFER modelling

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A comparison of the in vitro permeation of niacinamide in mammalian skin and in the Parallel Artificial Membrane Permeation Assay (PAMPA) model

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Studying transdermal patches using Skin PAMPA

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Studying semi-solid formulations of diclofenac using Skin PAMPA

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Application of Skin Pampa™ to Cosmetic Solvent Screening

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Using Skin-PAMPA For Transdermal Patch Testing

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Novel HT Method for Parallel Excipient/Vehicle Formulation Studies

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Assessment of Transdermal Penetration Enhancement by Topical Pharmaceutical Excipients Using Skin PAMPA Method

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Application of Skin PAMPA to Differentiate Between Topical Pharmaceutical Formulations of Ibuprofen

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Skin-PAMPA: Fast Prediction of Skin Penetration

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Skin-PAMPA: A New Method for Fast Prediction of Skin Penetration

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Developing Skin PAMPA for Transdermal Patches

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Evaluation of Optimized Caco-2 Permeability Measurements

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Human Jejunal Permeability Predicted from Caco-2 Assay - A Biophysical Model Applied to Fluoroquinolone Antimicrobials

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Very Thin PAMPA Membranes Indicate Higher Antipyrene Permeability but are Fragile and Contain Leaky Water Pores

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Prediction of in situ Blood-Brain Barrier Permeability using BBB PAMPA Model

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Drug Permeation in Skin PAMPA and Franz Cell Model

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Penetration Enhancer Effect of Sucrose Esters

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Novel in vitro PK (non-animal PK) Assay for Excipient/Vehicle Formulation Studies

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Prediction of Caco-2 pH-Dependent Permeability based on High Quality in vitro Training Set

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Consumables

GIT Test Compounds

110008

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Plate Lids, Pack of 10

110012

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Deepwell Plates Pack of 5

110023

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Stock Plate Pack of 10

110035

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Collector Plate (400 uL) Qty 10

110036

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Filter Plate Pack of 10

110037

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Test Tube 10 mL- 50

110075

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Test Tube 5 mL-50

110084

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Disposable Tips (200 uL) 1 box=Qty 960

110125

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Disposable Tips (20 uL) 1 box=Qty 960

110126

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Test Compounds Solubility

110130

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Collector Plate (500 uL) Qty 10

110132

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Acceptor Sink Buffer 250mL

110139

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Acceptor Sink Buffer (1L)

121041

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PRISMA HT 50mL 1

110151

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PRISMA LS 50 mL

110158

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PCR Plate, Pack of 5

110186

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Coated Stirrers (Quantity 1000) for use with Gut-Box

110211

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PRISMA HC 50mL

110238

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Stirwell Sandwich Pack of 5

110243

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High Profile Reservoir, Single Trough, Pack of 5

110252

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Low Profile Reservoir, Single Trough, Pack of 5

110253

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High Profile Reservoir 12 Trough, Pack of 5

110254

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Low Profile Reservoir 12 Trough, Pack of 2

110255

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UV Plates Pack of 10

110286

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Storage Plate

110323

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GIT-0 Lipid Pack of 5

110669

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BBB-1 Lipid Solution

110672

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BBB Test Compounds

110673

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Brain Sink Buffer BSB (250mL)

110674

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Skin Precoated Plate, Pack of 5

120657

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Skin Test Compounds

120687

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Skin Precoated Plate with Hydration Solution

120691

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Hydration Solution 250mL

120706

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Acceptor Sink Buffer (1L)

121041

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