Fiber Optic Advanced Training Course UK

Tuesday, 15th of September

8:15 – 8:45 Attendee Arrival

8:45 – 9:00 Introduction to Pion and personnel

9:00 – 10:15 Method Development Part 1: Getting started with the technology and basic considerations

• Introduction to Pion Fiber Optic instruments
• Principles and challenges of in situ concentration measurements
• Pathlength selections
• Generating good standard curve, “Blue Standards”
• Baseline correction algorithms
• 2nd derivative spectroscopy
• Calculation settings
• Sample Blank

10:15– 10:40 Break

10:40 – 11:25 Method Development Part 2: Advanced considerations

• Spectral shape analysis
• Blank and Reference Channel
• Can standard curve be prepared in different media
• Supersaturation considerations
• Media conversion experiments

11:25 – 12:10 Interactive Group Discussion #1: Fiber Optic Method Development. Working with changing background and molar absorptivity

12:10 – 1:00 Lunch (provided)

1:00 – 1:30 User Case Study #1 TBD

1:30 – 2:00 Zero intercept Method: Principles and Applications of ZIM

Dual-component dissolution

Monitoring for spectral consistency

Solubility of nanoparticles

Amorphous solubility vs. new phase formation

2:00 – 2:30 Group Discussion #2: Practical aspects of ZIM method implementation

2:30 – 2:50 Break

2:50 – 3:30 Advanced Tools: Dissolution Curve Analysis

• Relations between dissolution, solubility, particle size and hydrodynamics
• Intrinsic dissolution rate (IDR) measurements
• Predicting disk IDR from powder dissolution experiments
• Effective particle size estimation from the powder dissolution profile, prediction of Dissolution Number

3:30 – 4:45 Group Discussion #3: Continuation of ZIM and Dissolution Curve Analysis hands on practice

4:45 – 5:15 Q&A

5:30 – 8:00 Group social hour with follow up dinner

Wednesday, 16th of September

8:15 – 8:45 Attendee Arrival

8:45 – 9:30 Tools for understanding drug behavior – overview of Pion technology   

9:30 – 10:15 Towards in vivo predictive dissolution – flux measurements   

• Understanding the principles and driving forces of the flux
• Flux considerations
• Calculation and interpretation of flux data
• In vivo predictions using flux data

10:15 – 10:35 Break

10:35 – 11:05 User Case Study #2: TBD

11:05 – 11:50 Flux measurements: Analytical work behind the scene

• Detecting artifacts
• Membrane-excipient compatibility
• Ensuring membrane integrity
• Dual component flux
• Potential limitation of the flux measurement

11:50 – 12:20 User Case Study #3: TBD

12:20 – 1:20 Lunch (provided)

1:20 – 2:05 Group Discussion #4 Data Review and Basic Troubleshooting

2:05 – 2:50 Advanced Features of AuPRO Version 6

Dealing with more than 2 components—multicomponent regression analysis

Scatter modelling—can we estimate particle size from the shape of baseline?

Creating Reports

Standards for nanoparticles and colloids

Automating flux calculations

2:50 – 3:30 Q&A and Roundtable